Ampholyte-displacement chromatography for the separation of serum proteins.

The antibody fraction (less than 6ml) was added to immobilized enzyme (2ml of packed gel). The antibodies were recycled for 2h and the unattached antibodies washed from the gel for 1 h. Specific antibodies were eluted by washing with citric acid (0.1 M, pH2.2). Experiments marked with an asterisk were performed simultaneously. The molecular weights of immunoglobulin G and creatine kinase were taken as 150000 and 82000 respectively.